EEF2 (phospho-T56) polyclonal antibody

Product Specifications

Background

Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Elongation factor 2; EF-2; EEF2; EF2

Swiss Prot

P13639

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 20-70 of Human eEF2.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 95 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-EEF2 (T56) polyclonal antibody detects endogenous levels of EF-2 protein when phosphorylated at Thr56.

Applications Notes

Western blot (WB) analysis of p-EEF2 (T56) pAb at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (10ug) Lane3:The Liver tissue lysate of Rat (40ug) Lane4:The Liver tissue lysate of Mouse (40ug)