CrkL (phospho-Y207) polyclonal antibody

Product Specifications

Background

Crk, is a transformation-specific protein that induces elevation of cellular phosphotyrosine levels, but lacks tyrosine kinase activity itself. A second protein, Nck, consists solely of three SH3 domains and one SH2 domain, while GRB2 contains an SH2 domain flanked on both sides by SH3 domains. A member of this protein class, Crk-L, is encoded by a gene located on chromosome 22, band 11, centromeric of the chronic myelogenous leukemia breakpoint region. Crk-L encodes a 303 amino acid protein with one SH2 and two SH3 domains, and a predicted molecular weight of 36 kDa.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Crk L; Crk like protein; Crk-like protein; Crkl; Crkol; Oncogene CrkL

Swiss Prot

P46109

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human CrkL around the phosphorylation site of Tyrosine 207.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 39 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Crk-L (Y207) polyclonal antibody detects endogenous levels of Crk-L protein only when phosphorylated at Tyr207.

Applications Notes

Western blot (WB) analysis of p-CrkL (Y207) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:PMVEC whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug) Lane5:SGC7901 whole cell lysate (40ug)