C-Jun (phospho-T91) polyclonal antibody

Product Specifications

Background

The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein, along with c-Fos, is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE) . Two additional genes, Jun B and Jun D, have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

JUN; p39; V-jun avian sarcoma virus 17 oncogene homolog; Proto-oncogene c-Jun; AP1; Activator protein 1; Transcription factor AP-1

Swiss Prot

P05412

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Threonine 91.

Conjugation

Unconjugated

Related Pathways

MAPK Erk in Growth and Differentiation Pathway

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 43, 48 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-c-Jun (T91) polyclonal antibody detects endogenous levels of c-Jun protein only when phosphorylated at Thr91.

Applications Notes

Western blot (WB) analysis of p-c-Jun (T91) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate teated with UV Lane2:sp2/0 cell lysate lysate teated with UV Lane3:H9C2 cell lysate lysate teated with UV