Chk2 (phospho-T68) polyclonal antibody

Product Specifications

Background

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1..

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2; CHEK2; CDS1; CHK2; RAD53

Swiss Prot

O96017

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Chk2 around the phosphorylation site of Threonine 68.

Conjugation

Unconjugated

Related Pathways

Cell Cycle Control G1 S Checkpoint, Examples of Crosstalk Between Post-translational Modifications, Cell Cycle G2 M DNA Damage Signaling Pathway

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 62 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Chk2 (T68) polyclonal antibody detects endogenous levels of Chk2 protein when phosphorylated at Thr68.

Applications Notes

Western blot (WB) analysis of p-Chk2 (T68) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate treated with UV Lane2:Mouse kidney tissue lysate Lane3:H9C2 cell lysate treated with UV