Cdc6 (phospho-S54) polyclonal antibody
Product Specifications
Background
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cell division control protein 6 homolog; CDC6-related protein; Cdc18-related protein; HsCdc18; p62 (cdc6) ; HsCDC6; CDC6; CDC18L; p62 Cdc6
Swiss Prot
Q99741
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Cdc6 around the phosphorylation site of Serine 54.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 62 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Cdc6 (S54) polyclonal antibody detects endogenous levels of Cdc6 only when phosphorylated at Ser54.
Applications Notes
Western blot (WB) analysis of p-Cdc6 (S54) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with serum starvation Lane2:Raw264.7 cell lysate treated with serum starvation Lane3:H9C2 cell lysate treated with serum starvation
Host or Source
Rabbit
Available Sizes
Curated Selection
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