Cdc6 (phospho-S54) polyclonal antibody

Product Specifications

Background

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cell division control protein 6 homolog; CDC6-related protein; Cdc18-related protein; HsCdc18; p62 (cdc6) ; HsCDC6; CDC6; CDC18L; p62 Cdc6

Swiss Prot

Q99741

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Cdc6 around the phosphorylation site of Serine 54.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 62 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Cdc6 (S54) polyclonal antibody detects endogenous levels of Cdc6 only when phosphorylated at Ser54.

Applications Notes

Western blot (WB) analysis of p-Cdc6 (S54) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with serum starvation Lane2:Raw264.7 cell lysate treated with serum starvation Lane3:H9C2 cell lysate treated with serum starvation