AKT (phospho-S473) polyclonal antibody

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.

AKT1; AKT1; AKT2; AKT3; MGC9965; Oncogene AKT1; PKB alpha; PKB; PKB beta; PKBalpha; PRKBA; PRKBB; PRKBG; Protein kinase B; Protein kinase B beta; Protein kinase B gamma; Proto-oncogene c-Akt; RAC alpha; RAC; RAC gamma; RAC PK alpha; RAC PK beta; RAC

Phosphoinosotode Signaling, PI3K AKT signaling, Protein Kinase C Signaling, Immune Cell Signaling, Warburg Effect, Translational Contral elF4 and p70 S6 Kinase, Regulation of Microtubule Dynamics, Regulation of Microtubule Dynamics, T cell receptor signaling, Inhibition of Apoptosis, Insulin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint, Adherens Junction signaling, Mitochondrial Control of Apoptosis, MTOR signaling, ErbB HER Signaling

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of p-AKT (S473) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Mouse (40ug) Lane2:The Lung tissue lysate of Rat (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug) Lane5:L02 whole cell lysate (40ug)