TCEAL1 (E123) polyclonal antibody

Product Specifications

Background

TCEAL1 (transcription elongation factor A (SII) protein 3), also known as p21, SIIR or pp21, is a member of the TFS-II family. Transcription elongation factors of the TFS-II family are responsible for releasing RNA polymerase II (Pol II) from transcriptional arrest. DNA arresting sites can result in locked ternary complexes if elongating RNA polymerases are trapped. Transcription elongation factors function to activate the intrinsic RNA cleavage activity of RNA polymerases. This allows the RNA polymerase to cleave the nascent transcript, thereby forming a new 3’-terminus to resume elongation. TCEAL1 is a 157 amino acid nuclear protein that is ubiquitously expressed. TCEAL1 may exert its effects via protein-protein interactions with other transcriptional regulators rather than via direct binding of DNA.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

TCEA-like protein 1; TCEAL1; pp21; SIIR

Swiss Prot

Q15170

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 90-143 of Human TCEAL1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 18 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TCEAL1 (E123) polyclonal antibody detects endogenous levels of TCEAL1 protein.

Applications Notes

Western blot (WB) analysis of TCEAL1 (E123) pAb at 1:500 dilution Lane1:A2780 whole cell lysate (20ug) Lane2:PC3 whole cell lysate (20ug) Lane3:The Ovary tissue lysate of Rat (40ug) Lane4:3T3-L1 whole cell lysate (40ug)