Rab 5C (A153) polyclonal antibody

Product Specifications

Background

The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

RAB; member of RAS oncogene family like; RAB5C; RAB5C; member of RAS oncogene family; RAB5C; member RAS oncogene family; RAB5C; RAB5CL; RAB5L; RABL; Ras-related protein Rab-5C

Swiss Prot

P51148

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 130-180 of Human Rab 5C.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 24 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Rab 5C (A153) polyclonal antibody detects endogenous levels of Rab 5C protein.

Applications Notes

Western blot (WB) analysis of Rab 5C (A153) pAb at 1:1000 dilution Lane1:A375 whole cell lysate (40ug) Lane2:Hela whole cell lysate (30ug) Lane3:The Lung tissue lysate of Rat (40ug) Lane4:The Lung tissue lysate of Mouse (40ug)