Neuromedin-U (I174) polyclonal antibody

Product Specifications

Background

Neuromedin U (NmU) is a vertebrate peptide which stimulates uterine smooth muscle contraction and causes selective vasoconstriction. Like most other active peptides, it is proteolytically processed from a larger precursor protein. The mature peptides are 8 (NmU-8) to 25 (NmU-25) residues long and C-terminally amidated. The sequence of the C-terminal extremity of NmU is extremely well conserved across species.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

NmU 25; NMU

Swiss Prot

P48645

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids N-terminus of Human Neuromedin-U.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 20 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Neuromedin-U (I174) polyclonal antibody detects endogenous levels of Neuromedin-U protein.

Applications Notes

Western blot (WB) analysis of Neuromedin-U (I174) pAb at 1:500 dilution Lane1:CT26 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:K562 whole cell lysate (10ug) Lane4:HEK293T whole cell lysate (10ug)