PRIM1 (K413) polyclonal antibody
Product Specifications
Background
The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. PRIM1 is the small, 49 kDa primase subunit.The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
DNA primase small subunit; DNA primase 49 kDa subunit; p49; PRIM1
Swiss Prot
P49642
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 400-450 of Human PRIM1.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 50 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PRIM1 (K413) polyclonal antibody detects endogenous levels of PRIM1 protein.
Applications Notes
Western blot (WB) analysis of PRIM1 (K413) pAb at 1:1000 dilution Lane1:HepG2 whole cell lysate (20ug) Lane2:K562 whole cell lysate (20ug) Lane3:HEK293T whole cell lysate (20ug) Lane4:PC12 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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