DHS (L87) polyclonal antibody
Product Specifications
Background
Deoxyhypusine synthase (DHS) is crucial for the posttranslational formation of hypusine, a modification of a specific lysine residue in eukaryotic initiation factor 5A (eIF-5A) . Hypusine is formed by posttranslational modifications involving two enzymatic steps catalyzed by DHS and deoxyhypusine hydroxylase (DOHH) . eIF-5A is essential for eukaryotic cell proliferation. DHS, which belongs to the deoxyhypusine synthase family of proteins, is important for the first step in the hypusine biosynthesis pathway. It acts as a catalyst for the NAD-dependent oxidative cleavage of spermidine and the ensuing transfer of the butylamine moiety of spermidine to the eIF-5A protein, to create the intermediate deoxyhypusine residue.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Deoxyhypusine synthase; DHPS; DS
Swiss Prot
P49366
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 50-100 of Human DHS.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 45 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
DHS (L87) polyclonal antibody detects endogenous levels of DHS protein.
Applications Notes
Western blot (WB) analysis of DHS (L87) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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