DHS (L87) polyclonal antibody

Deoxyhypusine synthase (DHS) is crucial for the posttranslational formation of hypusine, a modification of a specific lysine residue in eukaryotic initiation factor 5A (eIF-5A) . Hypusine is formed by posttranslational modifications involving two enzymatic steps catalyzed by DHS and deoxyhypusine hydroxylase (DOHH) . eIF-5A is essential for eukaryotic cell proliferation. DHS, which belongs to the deoxyhypusine synthase family of proteins, is important for the first step in the hypusine biosynthesis pathway. It acts as a catalyst for the NAD-dependent oxidative cleavage of spermidine and the ensuing transfer of the butylamine moiety of spermidine to the eIF-5A protein, to create the intermediate deoxyhypusine residue.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of DHS (L87) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug)