ATP5A (K239) polyclonal antibody

Product Specifications

Background

Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes, F1, the hydrophilic catalytic core, and F0, the membrane-embedded protein channel. F1 consists of three α chains and three β chains, which are weakly homologous, as well as one γ chain, one δ chain and one ε chain. F0 consists of three subunits, a, b and c. The α chain of F1 is a regulatory subunit that contains 509 amino acids. Mitochondrial ATPase α chain (ATP5A) localizes to the mitochondria and catalyzes ATP synthesis.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ATP synthase subunit alpha, mitochondrial; ATP5A1; ATP5A; ATP5AL2; ATPM

Swiss Prot

P25705

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 210-260 of Human ATP5A.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ATP5A (K239) polyclonal antibody detects endogenous levels of ATP5A protein.

Applications Notes

Western blot (WB) analysis of ATP5A (K239) pAb at 1:1000 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug)