ACAT1 (K266) polyclonal antibody

Product Specifications

Background

ACAT-1 (acetyl-Coenzyme A acetyltransferase 1) is a mitochondrial enzyme involved in the formation and degradation of ketone bodies and is necessary for the proper metabolic processing of isoleucine. Rare defects in the gene encoding ACAT-1 lead to β-ketothiolase deficiency, which is characterized by ketoacidotic attacks. ACAT-2 (acetyl-Coenzyme A acetyltransferase 2) is considered a cytosolic protein and is crucial for cholesterol synthesis. Specifically, both Acetoacetyl-CoA specific thiolases, ACAT-1 and ACAT-2 catalyze the formation of acetoacetyl-CoA from two acetyl-CoA molecules. These enzymes are also capable of the reverse reaction, the cleavage of acetoacetyl-CoA into two acetyl-CoA molecules.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Acetyl-CoA acetyltransferase, mitochondrial; Acetoacetyl-CoA thiolase; ACAT1; ACAT; MAT; ACAT-1

Swiss Prot

P24752

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 235-286 of Human ACAT1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 45 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ACAT1 (K266) polyclonal antibody detects endogenous levels of ACAT1 protein.

Applications Notes

Western blot (WB) analysis of ACAT-1 (K266) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate (20ug) Lane2:HepG2 whole cell lysate (20ug) Lane3:The Lung tissue lysate of Rat (20ug) Lane4:The Lung tissue lysate of Mouse (10ug)