RPL28 (Y77) polyclonal antibody

Product Specifications

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

RPL 28; 60S ribosomal protein L28

Swiss Prot

P46779

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 50-100 of Human Ribosomal Protein L28.

Conjugation

Unconjugated

Applications

WB IHC IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 16 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Ribosomal Protein L28 (Y77) polyclonal antibody detects endogenous levels of Ribosomal Protein L28 protein.

Applications Notes

Western blot (WB) analysis of RPL28 (Y77) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:PC3 whole cell lysate (20ug) Lane3:A549 whole cell lysate (20ug) Lane4:The Lung tissue lysate of Rat (20ug) Lane5:The Kidney tissue lysate of Mouse (40ug)