Rb (T601) polyclonal antibody
Product Specifications
Background
The retinoblastoma tumor suppressor gene product, known as Rb or pRb, regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle, at G1/S, with transcriptional machinery that includes the heterodimeric E2F family. During G1, cyclin D (D1, 2, 3) -dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811 and Thr 821/Thr 826.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110; RB1
Swiss Prot
P06400
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 571-620 of Human Rb.
Conjugation
Unconjugated
Related Pathways
Cell Cycle Control G1 S Checkpoint
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 106 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Rb (T601) polyclonal antibody detects endogenous levels of Rb protein.
Applications Notes
Western blot (WB) analysis of Rb (T601) polyclonal antibody pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (20ug) Lane3:SK-OVCAR3 whole cell lysate (40ug) Lane4:786-O whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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