SEMA3A (P737) polyclonal antibody
Product Specifications
Background
Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co receptors, Neuropilin 1 and Plexin A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A containing region. Thus, Sema3A and its co receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Semaphorin-3A; Semaphorin III; Sema III; SEMAD; SEMA3A
Swiss Prot
Q14563
Reactivity
Human, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 702-756 of Human SEMA3A.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 89 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
SEMA3A (P737) polyclonal antibody detects endogenous levels of SEMA3A protein.
Applications Notes
Western blot (WB) analysis of SEMA3A (P737) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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