Integrin β3 (S778) polyclonal antibody

Product Specifications

Background

Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. The 16α and 8β subunits heterodimerize to produce more than 20 different receptors. Most Integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain Integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster Integrins by binding to adjacent Integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of Integrinmediated responses.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Integrin beta-3; Platelet membrane glycoprotein IIIa; Short name=GPIIIa; CD_antigen=CD61; ITGB3; GP3A; CD61; GPIIIa

Swiss Prot

P05106

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 751-800 of Human Integrin β3.

Conjugation

Unconjugated

Related Pathways

Tumor Angiogenesis, Contribution of ECM and Cytoskeletal Factors to EMT

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 87, 110 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Integrin β3 (S778) polyclonal antibody detects endogenous levels of Integrin β3 protein.

Applications Notes

Western blot (WB) analysis of Integrin β3 (S778) pAb at 1:500 dilution Lane1:BV2 whole cell lysate (40ug) Lane2:RAW264.7 whole cell lysate (40ug) Lane3:The Uterus tissue lysate of Rat (40ug) Lane4:THP-1 whole cell lysate (40ug) Lane5:A549 whole cell lysate (40ug) Lane6:PC3 whole cell lysate (40ug) Lane7:U-87MG whole cell lysate (40ug)