EIF2α (I45) polyclonal antibody

Product Specifications

Background

EIF2α, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2α controls the rate of tRNA translation. When eIF2α is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2α is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2A; EIF2S1

Swiss Prot

P05198

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 11-60 of Human eIF2α.

Conjugation

Unconjugated

Related Pathways

Translational Contral Regulation of elF2

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 38 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

EIF2α (I45) polyclonal antibody detects endogenous levels of eIF2α protein.

Applications Notes

Western blot (WB) analysis of eIF2α (I45) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:NIH-3T3 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug)