EIF2α (I45) polyclonal antibody
Product Specifications
Background
EIF2α, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2α controls the rate of tRNA translation. When eIF2α is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2α is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2A; EIF2S1
Swiss Prot
P05198
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 11-60 of Human eIF2α.
Conjugation
Unconjugated
Related Pathways
Translational Contral Regulation of elF2
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 38 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
EIF2α (I45) polyclonal antibody detects endogenous levels of eIF2α protein.
Applications Notes
Western blot (WB) analysis of eIF2α (I45) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:NIH-3T3 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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