Ezrin (G560) polyclonal antibody
Product Specifications
Background
Ezrin, Moesin and Radixin belong to a family of highly homologous Actinassociated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70% homology with Ezrin and are coexpressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cytovillin; Villin-2; Villin2; p81; EZR; VIL2
Swiss Prot
P15311
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 530-580 of Human Ezrin.
Conjugation
Unconjugated
Related Pathways
Contribution of ECM and Cytoskeletal Factors to EMT, B cell receptor signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 69, 81 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Ezrin (G560) polyclonal antibody detects endogenous levels of Ezrin protein.
Applications Notes
Western blot (WB) analysis of Ezrin (G560) pAb at 1:1000 dilution Lane1:Hela whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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