NGF (L82) polyclonal antibody
Product Specifications
Background
The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5) . These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Beta-nerve growth factor; Beta-NGF; NGFB
Swiss Prot
P01138
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 51-100 of Human NGF.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 34 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
NGF (L82) polyclonal antibody detects endogenous levels of Pro-NGF protein, this antibody does not cross-react with Mature-NGF.
Applications Notes
Western blot (WB) analysis of NGF (L82) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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