P-glycoprotein 1 (I583) polyclonal antibody

The MDR proteins (Mdrs) are members of a highly conserved superfamily of ATP-binding cassette transport proteins. Mdr functions as an energy-dependent efflux pump for structurally diverse agents ranging from ions to peptides. It is implicated in the development of the multiple drug resistance observed in human cancer cells following prolonged chemotherapy. The classic form of MDR is associated with an increase in the Mdr protein, but not all cases of MDR can be attributed to a rise in Mdr levels. Mdr-1 is an apical transmembrane protein that is an integral part of the blood-brain barrier and functions as a drug-transport pump transporting a variety of drugs from the brain back into the blood. In the human population, there are 15 polymorphisms in the Mdr-1 gene.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of P-glycoprotein 1 (I583) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug)