RUNX3 (R182) polyclonal antibody

Product Specifications

Background

RUNX family members are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX3 is expressed in cells of hematopoietic origin, including myeloid and B-cell lines and spleen. By playing a role in controlling the growth and differentiation of gastric epithelial cells, RUNX3 is a strong candidate as a gastric cancer tumor suppressor. Specifically, hypermethylation inactivates the gene encoding RUNX3. The detection of hypermethylation at multiple regions within the RUNX3 CpG island may aid in the diagnosis and risk assessment of gastric cancer.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Runt-related transcription factor 3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; CBF-alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEA2-alpha C; PEBP2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; RUNX3; AML2; CBFA3; PEBP2A3

Swiss Prot

Q13761

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 150-201 of Human RUNX3.

Conjugation

Unconjugated

Related Pathways

ESC Pluripotency and Differentiation Signaling Pathway

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 48 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RUNX3 (R182) polyclonal antibody detects endogenous levels of RUNX3 protein.

Applications Notes

Western blot (WB) analysis of RUNX3 (R182) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug)