RUNX3 (R182) polyclonal antibody
Product Specifications
Background
RUNX family members are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX3 is expressed in cells of hematopoietic origin, including myeloid and B-cell lines and spleen. By playing a role in controlling the growth and differentiation of gastric epithelial cells, RUNX3 is a strong candidate as a gastric cancer tumor suppressor. Specifically, hypermethylation inactivates the gene encoding RUNX3. The detection of hypermethylation at multiple regions within the RUNX3 CpG island may aid in the diagnosis and risk assessment of gastric cancer.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Runt-related transcription factor 3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; CBF-alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEA2-alpha C; PEBP2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; RUNX3; AML2; CBFA3; PEBP2A3
Swiss Prot
Q13761
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 150-201 of Human RUNX3.
Conjugation
Unconjugated
Related Pathways
ESC Pluripotency and Differentiation Signaling Pathway
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 48 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
RUNX3 (R182) polyclonal antibody detects endogenous levels of RUNX3 protein.
Applications Notes
Western blot (WB) analysis of RUNX3 (R182) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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