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CD34 (L257) polyclonal antibody

Product Specifications

Background

CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. CD34 antigen expression is highest in the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation consensus sites.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

CD34; Cd34; CD34 antigen; CD34 molecule; CD34; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; Mucosialin; OTTHUMP00000034733; OTTHUMP00000034734

Swiss Prot

P28906

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 225-270 of Human CD34.

Conjugation

Unconjugated

Applications

WB

Dilution

WB: 1:500~1:2000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 41 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CD34 (L257) polyclonal antibody detects endogenous levels of CD34 protein.

Applications Notes

Western blot (WB) analysis of CD34 (L257) pAb at 1:2000 dilution Lane1:The Kidney tissue lysate of Mouse (40ug) Lane2:The Kidney tissue lysate of Rat (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug)

Host or Source

Rabbit

Available Sizes

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