Cytokeratin 13 (E282) polyclonal antibody

Product Specifications

Background

Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Keratin, type I cytoskeletal 13; Cytokeratin-13; Cytokeratin 13; Cytokeratin13; Short name=CK-13; CK13; Keratin-13; Keratin13; Keratin 13; Short name=K13; KRT13

Swiss Prot

P13646

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 250-300 of Human Cytokeratin 13.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cytokeratin 13 (E282) polyclonal antibody detects endogenous levels of Cytokeratin 13 protein.

Applications Notes

Western blot (WB) analysis of Cytokeratin 13 (E282) pAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug)