TNF-β (H66) polyclonal antibody
Product Specifications
Background
Tumor necrosis factor b (TNFb), also known as lymphotoxin, is a pleiotropic cytokine. TNFa, also known as cachectin, is a smaller cytokine that binds to the same receptors, producing a vast array of effects similar to those of TNFb. TNFb and TNFa share 30% amino acid homology and have similar biological activities. TNFb is produced by activated lymphocytes, including CD4 (+) T helper cell type 1 lymphocytes, CD8 (+) lymphocytes and certain B lymphoblastoid cell lines. TNFa is produced by several different cell types, including lymphocytes, neutrophils and macrophages. TNFa and TNFb can modulate many immune and inflammatory functions, while having the ability to inhibit tumor growth.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Lymphotoxin-alpha; LT-alpha; TNF-beta; Lymphotoxin α; LTα; TNFβ; Tumor necrosis factor ligand superfamily member 1; LTA; TNFB; TNFSF1
Swiss Prot
P01374
Reactivity
Human, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 31-80 of Human TNF-β.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 22 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
TNF-β (H66) polyclonal antibody detects endogenous levels of TNF-β protein.
Applications Notes
Western blot (WB) analysis of TNF-β (H66) pAb at 1:500 dilution Lane1:The Testis tissue lysate of Rat (20ug) Lane2:A549 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (20ug) Lane4:PC3 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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