ABCG2 (N338) polyclonal antibody

Product Specifications

Background

ABCG2, is also known as placenta-specific ABC transporter and breast cancer resistance protein (BCRP1) . ABCG2 confers resistance for a variety of chemotherapeutic agents, including anthracyclines, mitoxantrone, bisantrene and topotecan. Under normal conditions, ABCG2 may serve a protective function by removing toxins from the cell, and plays an important role in regulating stem cell differentiation. ABCG2 is responsible for the side population (SP) phenotype and is widely expressed in a large variety of stem cells, making it an important stem cell marker. ABCG2 may have N-linked glycosylation and may dimerize in vivo. ABCG2 is abundantly expressed in placenta, liver, intestine and stem cells.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; Urate exporter; CD338; ABCP; BCRP; BCRP1; MXR

Swiss Prot

Q9UNQ0

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 302-356 of Human ABCG2.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 72 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ABCG2 (N338) polyclonal antibody detects endogenous levels of ATP-binding cassette sub-family G member 2 protein.

Applications Notes

Western blot (WB) analysis of ABCG2 (N338) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate