Ob-R (L662) polyclonal antibody

Product Specifications

Background

The first such recessive obesity mutation, the obese mutation (Ob), was identified in 1950. Mutation of Ob results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. It has been postulated that the Ob gene product may function as a component of a signaling pathway in adipose tissue that functions to regulate body fat depot size. The cloning and sequence analysis of the mouse Ob gene and its human homolog has recently been described. Ob encodes an adipose tissue-specific mRNA with a highly conserved 167 amino acid open reading frame. The predicted amino acid sequence is 84% identical between human and mouse and has the features of a secreted protein. A nonsense mutation in codon 105 has been found in the original congenic C57BL/6J Ob/Ob mouse strain. The Ob gene encodes the protein leptin.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Leptin receptor; LEP-R; HuB219 OB receptor; OB-R; CD295; LEPR; DB; OBR

Swiss Prot

P48357

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 650-700 of Human Ob-R.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 132 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Ob-R (L662) polyclonal antibody detects endogenous levels of Ob-R protein.

Applications Notes

Western blot (WB) analysis of Ob-R (L662) pAb at 1:500 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:SK-OVCAR3 whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug)