CA II (N229) polyclonal antibody
Product Specifications
Background
Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q21, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II; CA2
Swiss Prot
P00918
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 201-250 of Human CA II.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 28 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CA II (N229) polyclonal antibody detects endogenous levels of CA II protein.
Applications Notes
Western blot (WB) analysis of CA II (N229) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate (40ug) Lane2:The Kidney tissue lysate of Mouse (40ug) Lane3:The Brain tissue lysate of Rat (20ug) Lane4:HEK293T whole cell lysate (10ug)
Host or Source
Rabbit
Available Sizes
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