Cdc40 (S209) polyclonal antibody
Product Specifications
Background
Cdc40, also known as pre-mRNA-processing factor 17 (PRPF17) or EH-binding protein 3 (EHB3), is a 579 amino acid nuclear protein. Cdc40 is essential for the catalytic step II of the pre-mRNA splicing process, in which Cdc40 associates with the spliceosome C complex. Cdc40 contains seven WD repeats, which are important in protein-protein interactions. Cdc40 has sequence similarity to the yeast protein Prp17, which is involved in pre-mRNA splicing and cell cycle progression. The sequence similarity between the mammalian Cdc40 and the yeast Prp17 may indicate an additional role in cell cycle progression for mammalian Cdc40.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Pre-mRNA-processing factor 17; Cell division cycle 40 homolog; EH-binding protein 3; Ehb3; PRP17 homolog; hPRP17; CDC40; EHB3; PRP17; PRPF17
Swiss Prot
O60508
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 181-230 of Human Cdc40.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 65 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Cdc40 (S209) polyclonal antibody detects endogenous levels of Cdc40 protein.
Applications Notes
Western blot (WB) analysis of Cdc40 (S209) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (10ug) Lane2:MCF-7 whole cell lysate (10ug) Lane3:CT26 whole cell lysate (10ug) Lane4:The Brain tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
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