TWIK-1 (V322) polyclonal antibody

Product Specifications

Background

K+ channels are divided into three subclasses, reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS and 2TMS. Members of the 4TMS class contain two distinct pore regions, and include TASK, TREK, TRAAK and TWIK. TWIK-1 mRNA is expressed abundantly in brain and at lower levels in lung, kidney and skeletal muscle. The molecular weight of TWIK-1 in mouse brain is 40 kDa under reducing conditions. TWIK-2 shares low sequence homology with other mammalian family group members, and only 34% homology with TWIK-1. Human TWIK-2 is expressed in pancreas, placenta and heart, while mouse TWIK-2 is expressed in liver. TWIK-2 is inhibited by intracellular, but not extracellular, acidification.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Potassium channel subfamily K member 1; Inward rectifying potassium channel protein TWIK-1; Potassium channel KCNO1; KCNK1; HOHO1; KCNO1; TWIK1

Swiss Prot

O00180

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 301-350 of Human TWIK-1.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 38 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TWIK-1 (V322) polyclonal antibody detects endogenous levels of TWIK-1 protein.

Applications Notes

Western blot (WB) analysis of TWIK-1 (V322) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:PC12 whole cell lysate