POLR3A (P68) polyclonal antibody

Product Specifications

Background

POLR3A (polymerase (RNA) III (DNA directed) polypeptide A), also known as RPC1 or RPC155, is a 1,390 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase β chain family. Existing as the largest and catalytic core component of Pol III, POLR3A functions as a DNA-dependent RNA polymerase that catalyzes the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. The gene encoding POLR3A maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

DNA-directed RNA polymerase III subunit RPC1; RNA polymerase III subunit C1; EC=2.7.7.6; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; Short name=RPC155; RNA polymerase III subunit C160; POLR3A

Swiss Prot

O14802

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 41-90 of Human POLR3A.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 156 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

POLR3A (P68) polyclonal antibody detects endogenous levels of POLR3A protein.

Applications Notes

Western blot (WB) analysis of POLR3A (P68) pAb at 1:500 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:K562 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug)