POLR3A (P68) polyclonal antibody
Product Specifications
Background
POLR3A (polymerase (RNA) III (DNA directed) polypeptide A), also known as RPC1 or RPC155, is a 1,390 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase β chain family. Existing as the largest and catalytic core component of Pol III, POLR3A functions as a DNA-dependent RNA polymerase that catalyzes the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. The gene encoding POLR3A maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
DNA-directed RNA polymerase III subunit RPC1; RNA polymerase III subunit C1; EC=2.7.7.6; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; Short name=RPC155; RNA polymerase III subunit C160; POLR3A
Swiss Prot
O14802
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 41-90 of Human POLR3A.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 156 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
POLR3A (P68) polyclonal antibody detects endogenous levels of POLR3A protein.
Applications Notes
Western blot (WB) analysis of POLR3A (P68) pAb at 1:500 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:K562 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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