COP ζ1 (I48) polyclonal antibody

Product Specifications

Background

Membrane and vesicular trafficking in the early secretory pathway are mediated by non-Clathrin COP (coat protein) I-coated vesicles. COPI-coated vesicles mediate retrograde transport from the Golgi back to the ER and intra-Golgi transport. The cytosolic precursor of the COPI coat, the heptameric coatomer complex, is composed of two subcomplexes. The first consists of the COPB, COPG, COPD and COPZ1 subunits (also known as β-COP, γ-COP, δ-COP and ζ-1 COP, respectively), which are distantly homologous to AP Clathrin adaptor subunits. The second consists of the COPA, COPP and COPE subunits (also known as α-COP, COPP and ε-COP, respectively) .

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Coatomer subunit zeta-1; Zeta-1-coat protein; Zeta-1 COP; COPZ1; COPZ; CGI-120; HSPC181; COP ζ-1

Swiss Prot

P61923

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to the N-terminus of Human COP ζ1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 20 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

COP ζ1 (I48) polyclonal antibody detects endogenous levels of COP ζ1 protein.

Applications Notes

Western blot (WB) analysis of COP ζ1 (I48) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:HEK293T cell lysate Lane3:sp2/0 cell lysate Lane4:H9C2 cell lysate Lane5:PC12 cell lysate