DDX3 (S63) polyclonal antibody

Product Specifications

Background

DDX3 contains all of the motifs of the DEAD box family of RNA helicases, including the Asp/Glu/Ala/Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It is also involved in cell growth control and is deregulated in hepatitis virus associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ATP-dependent RNA helicase DDX3X; DEAD box protein 3, X-chromosomal; DEAD box, X isoform; Helicase-like protein 2; HLP2; DDX3X; DBX; DDX3

Swiss Prot

O00571

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 41-90 of Human DDX3.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 75 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

DDX3 (S63) polyclonal antibody detects endogenous levels of DDX3 protein.

Applications Notes

Western blot (WB) analysis of DDX3 (S63) pAb at 1:500 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug)