GPR103 (I310) polyclonal antibody

Product Specifications

Background

G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intracellular signal (G protein activation) . GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane-spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR103 is a 455 amino acid protein with highest expression in the brain, retina, trigeminal ganglion, hypothalamus and vestibular nucleus. In peripheral tissues, GPR103 is expressed only in the heart, kidney and testis. GPR103 may regulate adrenal function. A hypothalamic neuropeptide of the RFamide family (26RFa) acts as an endogenous ligand for GPR103.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Pyroglutamylated RFamide peptide receptor; AQ27; G-protein coupled receptor 103; Orexigenic neuropeptide QRFP receptor; SP9155; GPR103; QRFPR

Swiss Prot

Q96P65

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 178-334 of Human GPR103.

Conjugation

Unconjugated

Applications

WB IF

Dilution

WB: 1:500~1:1000 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 49 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

GPR103 (I310) polyclonal antibody detects endogenous levels of GPR 103 protein.

Applications Notes

Western blot (WB) analysis of GPR103 (I310) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:BV2 whole cell lysate (40ug)