GPR103 (I310) polyclonal antibody
Product Specifications
Background
G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intracellular signal (G protein activation) . GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane-spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR103 is a 455 amino acid protein with highest expression in the brain, retina, trigeminal ganglion, hypothalamus and vestibular nucleus. In peripheral tissues, GPR103 is expressed only in the heart, kidney and testis. GPR103 may regulate adrenal function. A hypothalamic neuropeptide of the RFamide family (26RFa) acts as an endogenous ligand for GPR103.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Pyroglutamylated RFamide peptide receptor; AQ27; G-protein coupled receptor 103; Orexigenic neuropeptide QRFP receptor; SP9155; GPR103; QRFPR
Swiss Prot
Q96P65
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 178-334 of Human GPR103.
Conjugation
Unconjugated
Applications
WB IF
Dilution
WB: 1:500~1:1000 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 49 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
GPR103 (I310) polyclonal antibody detects endogenous levels of GPR 103 protein.
Applications Notes
Western blot (WB) analysis of GPR103 (I310) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:BV2 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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