MED18 (V102) polyclonal antibody

Product Specifications

Background

In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II) . The mediator complex is one such multi-protein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med18 (mediator complex subunit 18), also known asp28b, is a 208 amino acid protein that localizes to nucleus and exists as a component of the mediator complex. Working in tandem with several other proteins, including Med8 and Med25, Med18 serves as a scaffold for the assembly of a functional preinitiation complex with Pol II and general transcription factors, thereby activating the transcription of Pol II-dependent genes.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Mediator of RNA polymerase II transcription subunit 18; Mediator complex subunit 18; p28b; MED18

Swiss Prot

Q9BUE0

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 72-120 of Human MED18.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 24 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MED18 (V102) polyclonal antibody detects endogenous levels of MED18 protein.

Applications Notes

Western blot (WB) analysis of MED18 (V102) pAb at 1:1000 dilution Lane1:HepG2 whole cell lysate (20ug) Lane2:SGC7901 whole cell lysate (20ug) Lane3:The Brain tissue lysate of Rat (40ug) Lane4:The Brain tissue lysate of Mouse (40ug)