MED18 (V102) polyclonal antibody

In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II) . The mediator complex is one such multi-protein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med18 (mediator complex subunit 18), also known asp28b, is a 208 amino acid protein that localizes to nucleus and exists as a component of the mediator complex. Working in tandem with several other proteins, including Med8 and Med25, Med18 serves as a scaffold for the assembly of a functional preinitiation complex with Pol II and general transcription factors, thereby activating the transcription of Pol II-dependent genes.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of MED18 (V102) pAb at 1:1000 dilution Lane1:HepG2 whole cell lysate (20ug) Lane2:SGC7901 whole cell lysate (20ug) Lane3:The Brain tissue lysate of Rat (40ug) Lane4:The Brain tissue lysate of Mouse (40ug)