Aldolase B (W148) polyclonal antibody

Product Specifications

Background

Aldolase A is one of the most highly conserved enzymes known, with only about 2% of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagons and has been implicated in hereditary fructose intolerance disease. Aldolase C is 36 kDa polypeptide that is exclusively expressed in Purkinje cells. Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ALDOB; ALDOB; ALDB; Liver-type aldolase; Fructose-bisphosphate aldolase B

Swiss Prot

P05062

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 121-170 of Human Aldolase B.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:2000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 40 to 45 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Aldolase B (W148) polyclonal antibody detects endogenous levels of Aldolase B protein.

Applications Notes

Western blot (WB) analysis of Aldolase B (W148) pAb at 1:2000 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:The Kidney tissue lysate of Rat (10ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (20ug) Lane5:SGC7901 whole cell lysate (20ug)