POLR2E (L58) polyclonal antibody
Product Specifications
Background
This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
DNA-directed RNA polymerases I, II, and III subunit RPABC1; RNA polymerases I, II, and III subunit ABC1; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4; POLR2E
Swiss Prot
P19388
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 30-80 of Human POLR2E.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 25 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
POLR2E (L58) polyclonal antibody detects endogenous levels of POLR2E protein.
Applications Notes
Western blot (WB) analysis of POLR2E (L58) polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug) Lane5:786-O whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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