CLC-4 (E254) polyclonal antibody

Product Specifications

Background

The family of voltage-dependent chloride channels (CLCs) regulate cellular trafficking of chloride ions, a critical component of all living cells. CLCs regulate excitability in muscle and nerve cells, aid in organic solute transport and maintain cellular volume. The genes encoding human CLC-1 through CLC-7 map to chromosomes 7, 3q26, 4q32, Xp22, Xp11, 1p36 and 16p13, respectively. CLC-1 is highly expressed in skeletal muscle. Mutations in the gene encoding CLC-1 lead to myotonia, an inheritable disorder characterized by muscle stiffness and renal salt wasting. CLC-2 is highly expressed in the epithelia of several organs including lung, which suggests CLC-2 may be a possible therapeutic target for cystic fibrosis. CLC-3 expression is particularly abundant in neuronal tissue, while CLC-4 expression is evident in skeletal and cardiac muscle as well as brain.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

H (+) /Cl (-) exchange transporter 4; Chloride channel protein 4; ClC-4; Chloride transporter ClC-4; CLCN4; CLC 4; CLC4

Swiss Prot

P51793

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 240-280 of Human CLC-4.

Conjugation

Unconjugated

Applications

WB IF

Dilution

WB: 1:500~1:1000 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 85 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CLC-4 (E254) polyclonal antibody detects endogenous levels of CLC-4 protein.

Applications Notes

Western blot (WB) analysis of CLC-4 (E254) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:Raw264.7 cell lysate Lane3:PC12 cell lysate