Calponin 2 (D156) polyclonal antibody

Product Specifications

Background

Calponin, a 34 kDa protein, regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an actin- and tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-actin and inhibition of the actin-activated MgATPase activity of phosphorylated myosin.Both properties are lost following phosphorylation (primarily at serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin), and Calponin 3 (acidic Calponin) are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Calponin-2; Calponin H2, smooth muscle; Neutral calponin; CNN2

Swiss Prot

Q99439

Reactivity

Human, Mouse

Immunogen

Synthetic peptide, corresponding to amino acids 122-170 of Human Calponin 2.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 33 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Calponin 2 (D156) polyclonal antibody detects endogenous levels of Calponin 2 protein.

Applications Notes

Western blot (WB) analysis of Calponin 2 (D156) pAb at 1:500 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (20ug) Lane3:L02 whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug)