Arfaptin 1 (K314) polyclonal antibody
Product Specifications
Background
Arfaptin 1 is an ARF binding protein that inhibits PLD activation, vesicular trafficking and secretion. In the present report, we show that arfaptin 1 interacts with 'high speed' membranes independently of ARF. However, addition of myristoylated ARF3 (myrARF3) increases the association of arfaptin 1 with the membranes, suggesting that arfaptin 1 and ARF form a complex on the Golgi. Utilizing several deletion mutants of arfaptin 1 it is shown that the association of arfaptin 1 with myrARF3 is mediated via two binding sites on arfaptin 1. These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Arfaptin-1; ADP-ribosylation factor-interacting protein 1; ARFIP1; Arfaptin1
Swiss Prot
P53367
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 300-350 of Human Arfaptin 1.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 40 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Arfaptin 1 (K314) polyclonal antibody detects endogenous levels of Arfaptin 1 protein.
Applications Notes
Western blot (WB) analysis of Arfaptin 1 (K314) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:SP2/0 cell lysate Lane3:PC12 cell lysate Lane4:Rat muscle tissue lysate
Host or Source
Rabbit
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