ARF4 (E106) polyclonal antibody

Product Specifications

Background

The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with Golgi membranes and the association of actin to the Golgi via rho family-dependent G-protein localization (Rac, CDC42) and WASP/Arp2/3 complexes. Additionally, ARFs play a central role in maintenance of organelle integrity, assembly of coat proteins, and activation of phospholipase D. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6) ; members of each class share a common gene organization. The human ARF1 gene maps to chromosome 1q42, contains 5 exons and 4 introns, and encodes a 181 amino acid protein.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ADP ribosylation factor 2; ADP ribosylation factor 4; ARF2

Swiss Prot

P18085

Reactivity

Human, Mouse

Immunogen

Synthetic peptide, corresponding to amino acids 80-130 of Human ARF4.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 21 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ARF4 (E106) polyclonal antibody detects endogenous levels of ARF4 protein.

Applications Notes

Western blot (WB) analysis of ARF4 (E106) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:L02 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug)