RPL27A (V140) polyclonal antibody
Product Specifications
Background
Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L27, also known as RPL27, is a 136 amino acid protein belonging to the ribosomal protein L27e family exists as a component of the 60S subunit, possibly playing a role in protein translation. Like most ribosomal proteins, Ribosomal Protein L27 exists as multiple processed pseudogenes that are scattered throughout the genome. Considered a novel candidate housekeeping gene, the gene encoding Ribosomal Protein L27 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
60S ribosomal protein L27a
Swiss Prot
P46776
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 120-160 of Human Ribosomal Protein L27A.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 17 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Ribosomal Protein L27A (V140) polyclonal antibody detects endogenous levels of Ribosomal Protein L27A protein.
Applications Notes
Western blot (WB) analysis of RPL27A (V140) pAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:The Brain tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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