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RPL27A (V140) polyclonal antibody

Product Specifications

Background

Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L27, also known as RPL27, is a 136 amino acid protein belonging to the ribosomal protein L27e family exists as a component of the 60S subunit, possibly playing a role in protein translation. Like most ribosomal proteins, Ribosomal Protein L27 exists as multiple processed pseudogenes that are scattered throughout the genome. Considered a novel candidate housekeeping gene, the gene encoding Ribosomal Protein L27 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

60S ribosomal protein L27a

Swiss Prot

P46776

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 120-160 of Human Ribosomal Protein L27A.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 17 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Ribosomal Protein L27A (V140) polyclonal antibody detects endogenous levels of Ribosomal Protein L27A protein.

Applications Notes

Western blot (WB) analysis of RPL27A (V140) pAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:The Brain tissue lysate of Rat (40ug)

Host or Source

Rabbit

Available Sizes

Curated Selection

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