DnaJB4 (R120) polyclonal antibody
Product Specifications
Background
DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein. The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis. The proteins contain cysteine rich regions that are composed of zinc fingers that form a peptide binding domain responsible for the chaperone function. DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. DnaJB4 (DnaJ homolog subfamily B member 4), also known as HLJ1, is expressed in skeletal muscle, heart and pancreas, and lower expression in brain, placenta and liver.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
DjB4; DnaJ (Hsp40) homolog subfamily B member 4; DnaJ homolog subfamily B member 4; DnaJ like heat shock protein 40; DNAJB 4; DNAJW; Heat shock 40 kDa protein 1 homolog; Heat shock protein 40 homolog; HLJ 1; HLJ1; HSP40 homolog
Swiss Prot
Q9UDY4
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 90-132 of Human DnaJB4.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 40 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
DnaJB4 (R120) polyclonal antibody detects endogenous levels of DnaJB4 protein.
Applications Notes
Western blot (WB) analysis of DnaJB4 (R120) pAb at 1:500 dilution Lane1:PANC1 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:The Heart tissue lysate of Mouse (40ug) Lane5:The Heart tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
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