Histone H2A.X (S130) polyclonal antibody

Product Specifications

Background

H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone H2AX; H2a/x; Histone H2A.X; H2AFX; H2AX

Swiss Prot

P16104

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 100-150 of Human Histone H2A.X.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Histone Lysine Methylation Pathway

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 15 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Histone H2A.X (S130) polyclonal antibody detects endogenous levels of Histone H2A.X protein.

Applications Notes

Western blot (WB) analysis of Histone H2A.X (S130) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:3T3-L1 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug)