CA IX (D82) polyclonal antibody
Product Specifications
Background
CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA9, CA12 and CA14 genes, which map to chromosomes 9p13.3, 15q22 and 1q21, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression. CA IX is specifically expressed in clear-cell renal carcinomas, whereas CA XII is highly expressed in normal tissues, such as kidney, colon and pancreas.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Carbonic anhydrase 9; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250 pMW1; CA9; G250; MN
Swiss Prot
Q16790
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 50-104 of Human CA IX.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 50 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CA IX (D82) polyclonal antibody detects endogenous levels of CA IX protein.
Applications Notes
Western blot (WB) analysis of CA IX (D82) pAb at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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