Sam 68 (Y145) polyclonal antibody

Product Specifications

Background

SAM68 is recruited and tyrosine phosphorylated by several receptor systems, and once it is phosphorylated, it functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins.It represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. It also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

KH domain-containing; RNA-binding; signal transduction-associated protein 1; GAP-associated tyrosine phosphoprotein p62; Src-associated in mitosis 68 kDa protein; Sam68; p21 Ras GTPase-activating protein-associated p62; p68; KHDRBS1; SAM68

Swiss Prot

Q07666

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 120-170 of Human Sam 68.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 48 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Sam 68 (Y145) polyclonal antibody detects endogenous levels of Sam 68 protein.

Applications Notes

Western blot (WB) analysis of Sam 68 (Y145) pAb at 1:1000 dilution Lane1:HCC827 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug)